Initiation of Translation in Bacteria and Chloroplasts

Relative rates of protein synthesis in bacteria generally depend on the number of copies of a messenger RNA (mRNA) and the efficiency of their loading with ribosomes. Translation initiation involves the multi-stage assembly of the ribosome on the mRNA to begin protein synthesis. In bacteria, the small ribosomal subunit (30S) and mRNA form a complex that can be supported by RNA-protein and RNA-RNA interactions and is extensively modulated by mRNA folding. The initiator transfer RNA (tRNA) and large ribosomal subunit (50S) are recruited with aid of three initiation factors (IFs). Equivalent translation initiation processes occur in chloroplasts due to their endosymbiotic origin from photosynthetic bacteria. This review first summarizes the molecular basis of translation initiation in bacteria, highlighting recent insight into the initial, intermediate and late stages of the pathway obtained by structural analyses. The molecular basis of chloroplast translation initiation is then reviewed, integrating our mechanistic understanding of bacterial gene expression supported by detailed in vitro experiments with data on chloroplast gene expression derived primarily from genetic studies.