Related Experiment Video
Updated: May 13, 2025
Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia
Published on: June 28, 2018
Clinical value of dysregulated miR-125b-5p in severe pneumonia children
Meiqin Zhu1, Ziyan Lu2, Xingjuan Liao3
1Department of Respiratory, The Fourth Affiliated Hospital of Jiangsu University (Zhenjiang Maternal and Child Health Hospital), Zhenjiang, 212001, China.
Insights
Reduced microRNA-125b-5p (miR-125b-5p) levels in children with severe pneumonia indicate a potential diagnostic marker. Lower miR-125b-5p also independently predicts a poorer prognosis in these young patients.
Area of Science:
- Pediatric critical care medicine
- Molecular diagnostics
- Biomarker discovery
Background:
- Severe pneumonia is a leading cause of mortality in young children.
- MicroRNA-125b-5p (miR-125b-5p) is implicated in lung diseases, but its role in pediatric severe pneumonia is unestablished.
Purpose of the Study:
- To investigate the expression levels and clinical significance of miR-125b-5p in children diagnosed with severe pneumonia.
Main Methods:
- Serum miR-125b-5p expression was quantified using qRT-PCR in 96 pneumonia and 127 severe pneumonia children (aged 2-10 years).
- Receiver operator characteristic (ROC) curve analysis was used for diagnostic evaluation.
- Kaplan-Meier and multivariate Cox regression analyses assessed prognostic value.
Main Results:
- Serum miR-125b-5p levels were significantly lower in severe pneumonia cases compared to pneumonia cases (AUC=0.9267).
- Low miR-125b-5p expression correlated with decreased survival rates (P=0.033).
- Reduced miR-125b-5p (HR=0.301) and increased procalcitonin (PCT) (HR=2.631) were independent risk factors for poor prognosis.
Conclusions:
- Decreased miR-125b-5p serves as a potential diagnostic biomarker for severe pneumonia in children.
- Reduced miR-125b-5p is an independent predictor of poor prognosis in pediatric severe pneumonia.
Background:
Severe pneumonia is an important contributor to the high mortality of sick young children. The microRNA-125b-5p (miR-125b-5p), which is widely involved in various cancers, is closely related to a variety of lung diseases. However, its role in severe pneumonia children remains to be studied.
Objective:
This study focused on the expression and clinical value of miR-125b-5p in severe pneumonia children.
Materials And Methods:
The study subjects included 96 pneumonia children and 127 severe pneumonia children. These children were aged between 2-10 years. The expression level of serum miR-125b-5p was assessed by qRT-PCR. The receiver operator characteristic (ROC) curve was employed to identify severe pneumonia children from pneumonia individuals. Kaplan-Meier curve was plotted based on follow-up results and multivariate Cox regression analysis was applied to evaluate the contribution of miR-125b-5p to poor prognostic in severe pneumonia children.
Results:
MiR-125b-5p was remarkedly reduced in severe pneumonia children compared to pneumonia individuals. The area under the curve (AUC) was 0.9267 and the sensitivity and specificity were 84.25% and 89.58%, respectively. The accumulative survival rate in low miR-125b-5p group showed a remarkable decrease compared to the high miR-125b-5p group (P = 0.033). Increased procalcitonin (PCT, HR: 2.631, 95% CI: 1.029-6.732, P = 0.043) and reduced miR-125b-5p (HR: 0.301, 95% CI: 0.110-0.826, P = 0.020) were found to be related to the poor prognosis in severe pneumonia children.
Conclusion:
The reduced miR-125b-5p was an underlying diagnostic indicator of severe pneumonia and was an independent risk factor of poor prognosis in severe pneumonia children.
Related Concept Videos
01:30Pneumonia III: Complications and Assessment
01:28Pneumonia IV: Management
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
01:30Pneumonia I: Introduction
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...