Fast response cathodic electrochemiluminescence sensor based on closed bipolar electrode for point-of-care blood...

Area of Science:

• Electrochemistry
• Biosensors
• Analytical Chemistry

Background:

• Glucose detection is crucial for diabetes management and metabolic disorder monitoring.
• Electrochemical methods are common for glucose detection but face challenges like interference and electrode fouling.
• Existing methods require improvements for sensitivity, stability, and reduced interference.

Purpose of the Study:

• To develop a fast-response cathodic electrochemiluminescence (ECL) glucose biosensor.
• To create a closed bipolar electrode (BPE) system that minimizes external interference.
• To provide a cost-effective and reliable method for point-of-care glucose testing.

Main Methods:

• Utilized a closed bipolar electrode (BPE) system with separate sensing and reporting cells.
• Employed Tris(2,2'-bipyridyl) ruthenium as the luminophore and K2S2O8 as the co-reagent for ECL generation.
• Modified a commercial test strip with glucose oxidase (GOx) and a mediator for glucose detection in the sensing cell.

Main Results:

• Achieved sensitive glucose detection with a limit of detection of 3.8 mM and a linear range of 4-12 mM.
• Demonstrated a fast response time and good correlation with glucose quantification in human serum samples.
• Successfully quantified glucose up to 18 mM in buffer solutions, showing comparability to commercial blood glucose meters.

Conclusions:

• The developed BPE-based ECL biosensor provides a simple, cost-effective strategy for point-of-care blood glucose testing.
• The system effectively avoids external interference, enhancing the reliability of glucose measurements.
• This technique presents promising opportunities for various sensing applications beyond glucose monitoring.