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Updated: May 14, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
Published on: March 2, 2012
Trends development and applications on electrophoresis techniques of slab gel, capillary, microchip/microfluidic
1College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518055, PR China; Institute for Advanced Study, Shenzhen University, Shenzhen 518055, PR China.
Abstract:
This review describes the various electrophoresis techniques involved in slab gel, capillary, microchip, and isotachophoresis. Each technique offers distinct advantages and limitations in terms of resolution, sensitivity, speed, capacity, and cost of resources. While the manuscript provides an overview of the setup methods for these electrophoresis techniques, it also evaluates their unique characteristics and summarizes a range of analytical applications, including environmental monitoring, proteomics and genomics analysis, clinical diagnostics, pharmaceutical analysis, and biochemical research. This review contributes to the future directions of available electrophoresis techniques and aids knowledge seekers or practitioners in selecting the most appropriate methods for their specific analytical needs. This review highlights the strengths and potential applications of each technique, providing insights into advancing analytical methodologies and exploring emerging trends across scientific disciplines.
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