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Updated: Jul 31, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
Published on: June 15, 2012
Efficient extraction of Macadamia nut oil and Palmitoleic acid enrichment with freeze-dried microencapsulation
Zhiqing Liu1, Hansen Chen1, Juntai Chen1
1Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou, Guangdong 510640, China.
Abstract:
The extraction process of macadamia nut oil (MNO) was optimized in this study, focusing on enhancing the enrichment and encapsulation efficiency of palmitoleic acid (PA). Three different methods for extracting MNO-ultrasound-assisted hydrolase extraction, organic solvent extraction, and physical cold pressing-were compared, and the properties of MNO were analyzed. A combination of low-temperature solvent crystallization and molecular distillation was used to separate and purify PA. Sodium caseinate and d-lactose were used as wall materials to prepare PA microcapsules via freeze-drying, and the key factors influencing their performance were investigated. Under optimal conditions, the MNO extraction yield was 80.10 ± 2.45 %, with a PA content of 40.54 ± 2.55 % and a PAM encapsulation efficiency of 80.21 ± 3.22 %. This study provides novel technical approaches and theoretical foundations for the industrial extraction of MNO, enrichment of PA, and their microencapsulation, paving the way for potential large-scale production and industrial applications.

