Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.1K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.1K
Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

2.2K
Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
2.2K
Centrosome Duplication02:25

Centrosome Duplication

3.8K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
3.8K
Spindle Assembly02:50

Spindle Assembly

3.4K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.4K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.2K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.2K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

5.5K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Profile: Dr. Harshita Kasera.

Cytoskeleton (Hoboken, N.J.)·2025
Same author

Overreliance on Plasmodium 18S rRNA gene for malaria molecular diagnosis-inferences from systematic review.

Malaria journal·2025
Same author

Exploring the Therapeutic Potential of Lipid Nanocarriers in Psoriasis Management: Advances and Applications.

AAPS PharmSciTech·2025
Same author

Ensuring integrity and security of medical image transmission in IoMT using highly imperceptible and robust watermarking approach.

Scientific reports·2025
Same author

Bacterial endotoxin-lipopolysaccharide role in inflammatory diseases: An overview.

Iranian journal of basic medical sciences·2025
Same author

Comparative anti-cancer properties of carene isoforms induced apoptotic cell death in stomach and lung cancer cell lines.

Naunyn-Schmiedeberg's archives of pharmacology·2025

Related Experiment Video

Updated: May 13, 2025

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.0K

PLK4 Homodimerization is Required for CEP152 Centrosome Localization and Spindle Organization.

Harshita Kasera1, Srishti Sanghi1, Priyanka Singh1

  • 1Department of Bioscience & Bioengineering, Indian Institute of Technology Jodhpur, NH 62, Nagaur Road, Karwar 342037, Jodhpur, Rajasthan, India.

Journal of Molecular Biology
|April 13, 2025
PubMed
Summary

Polo-Like Kinase 4 (PLK4) homodimerization is crucial for centrosome function and stability. Disruption of this process, seen in a cancer variant, impairs CEP152 recruitment, leading to spindle defects and reduced cell viability.

Keywords:
CEP152CEP192PLK4centriolecentrosome

More Related Videos

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.1K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.2K

Related Experiment Videos

Last Updated: May 13, 2025

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.0K
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.1K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Biology

Background:

  • Polo-Like Kinase 4 (PLK4) is a centrosome-specific kinase.
  • PLK4 homodimerizes via its cryptic polo-box (CPB) region, leading to autophosphorylation and degradation.
  • The CPB region also mediates interactions with centrosome recruiters CEP152 and CEP192.

Purpose of the Study:

  • To investigate the role of PLK4 homodimerization in the CEP192-CEP152 network.
  • To characterize a cancer-associated PLK4 variant disrupting CPB-mediated interactions.
  • To understand the functional consequences of impaired PLK4 homodimerization on centrosome integrity and cell viability.

Main Methods:

  • Identification and characterization of a truncated PLK4 variant.
  • Analysis of PLK4 homodimerization and interaction with CEP152/CEP192 using the variant.
  • Assessment of CEP152 and pericentrin localization at centrosomes during S-phase.
  • Evaluation of spindle organization and cell viability during M-phase.

Main Results:

  • A cancer PLK4 variant truncates the protein, abolishing homodimerization and interaction with CEP152/CEP192.
  • PLK4 homodimerization is essential for maintaining CEP152 levels at centrosomes during S-phase.
  • Expression of the PLK4 homodimerization mutant leads to reduced CEP152 and pericentrin levels.
  • Impaired PLK4 homodimerization results in unfocused spindles and decreased cell viability.

Conclusions:

  • PLK4 homodimerization and CEP152 recruitment exhibit a cross-dependency for proper centrosome function.
  • Disruption of PLK4 homodimerization compromises centrosome integrity and contributes to cancer-associated defects.
  • This highlights a critical regulatory mechanism at the centrosome that is altered in cancer.