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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: May 13, 2025

Synthetic Spider Silk Production on a Laboratory Scale

Synthetic Spider Silk Production on a Laboratory Scale

Published on: July 18, 2012

Spider Eye Development Editing and Silk Fiber Engineering Using CRISPR-Cas.

Edgardo Santiago-Rivera¹, Thomas Scheibel^1,2,3,4,5

¹Department of Biomaterials, University of Bayreuth, Prof.-Rüdiger-Borman Strasse 1, 95448, Bayreuth, Germany.

Angewandte Chemie (International Ed. in English)

|April 14, 2025

Summary

This summary is machine-generated.

CRISPR gene editing was successfully applied to spiders for the first time, creating gene knock-out and knock-in mutations in offspring. This research enables genetic modification in spiders for developmental genetics and material science applications.

Keywords:

Eye‐loss Fluorescent silk fibers Genotype Knock‐in Knock‐out Major ampullate silk

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Last Updated: May 13, 2025

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Area of Science:

Genetics
Developmental Biology
Material Science

Background:

CRISPR-Cas9 is a precise genome editing tool with broad applications.
CRISPR-based gene editing had not been previously reported in spiders.

Purpose of the Study:

To demonstrate the feasibility of CRISPR-Cas9 gene editing in spiders.
To investigate gene function and enable silk protein functionalization in spiders.

Main Methods:

CRISPR-mediated microinjection was performed in parental spiders.
Knock-out (KO) and knock-in (KI) mutations were induced in offspring.
The sine oculis gene was targeted for KO, and a fluorescent protein was targeted for KI.

Main Results:

KO of the sine oculis gene resulted in complete eye loss in offspring, confirming its role in eye development.
KI of monomeric red fluorescent protein (mRFP-KI) into a silk gene produced red fluorescent silk fibers.
Silk assembly was not affected by the KI mutation, demonstrating functionalization feasibility.

Conclusions:

CRISPR-Cas9 gene editing is now applicable to spiders.
This technique allows for the study of developmental genetics and the creation of novel silk materials.