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Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function
Ali Asghar Zarei1, Sara Keshtgar1, Masoud Haghani1
1Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Background:
Calcium enters human sperm through the "Cation Channel of Sperm" (CatSper), while potassium ions exit via the sperm potassium channel (KSper). These two channels regulate intracellular calcium concentration ([Ca2+]i) and membrane potential. Our study aims to investigate and compare the contributions of these channels in capacitated sperm function.
Results:
NNC and quinidine significantly decreased progressive sperm motility (P=0.001) and reduced sperm kinematics (P=0.001). NNC but not quinidine significantly decreased sperm survival (P=0.001), reduced [Ca2+]i in live spermatozoa (P=0.05), and induced the acrosomal reaction (P=0.012).
Conclusion:
Inhibition of KSper without effect on [Ca2+]i can inhibit sperm motility and increase mortality rate. It seems that the function of KSper is as vital as CatSper in human sperm physiology.
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