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Updated: May 13, 2025
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
Published on: August 12, 2020
The Role of Neutrophil CD11b Compared to Neutrophil CD64 as an Early Diagnostic, Monitoring, and Prognostic Sepsis
Heba E Hashem1, Wafaa O Ahmed1, Safeya H Hassan1
1Clinical Pathology Department, Ain Shams University Hospitals, Cairo, Egypt.
Insights
Neutrophil CD64 (nCD64) shows superior diagnostic performance for neonatal sepsis compared to CD11b. Combining nCD64 with hs-CRP offers a rapid and accurate diagnostic tool for neonatal intensive care units (NICUs).
Area of Science:
- Neonatal Medicine
- Immunology
- Biomarker Discovery
Background:
- Neonatal sepsis poses significant risks, including mortality, neurodevelopmental disabilities, and lifelong morbidities.
- Early diagnosis and treatment are critical to mitigate these severe consequences.
Purpose of the Study:
- To evaluate the diagnostic and prognostic performance of CD11b as a sepsis biomarker in neonates.
- To compare CD11b's efficacy against neutrophil CD64 (nCD64) and conventional sepsis parameters.
Main Methods:
- A cohort of 211 neonates (101 controls, 110 sepsis) was studied in Egyptian NICUs.
- Sepsis screening included CBC, hs-CRP, blood cultures, and flow cytometry for CD64 and CD11b on neutrophils.
Main Results:
- Neutrophil CD64 percentage (nCD64%) demonstrated high diagnostic performance (92.8% sensitivity, 90.8% specificity, AUC=0.894).
- CD11b showed poor diagnostic utility (CD11b% AUC=0.405, CD11b MFI AUC=0.144).
- Combining hs-CRP and nCD64% yielded the highest diagnostic accuracy (93.9% sensitivity, 97.2% specificity, AUC=0.938).
Conclusions:
- Neutrophil CD64% is a superior biomarker for neonatal sepsis compared to CD11b and hs-CRP alone.
- Combined measurement of nCD64% and hs-CRP provides a highly accurate and rapid diagnostic method for neonatal sepsis.
- Neutrophil CD64 can be routinely applied in NICUs for improved sepsis management.
Abstract:
Background: Early diagnosis and treatment of neonatal sepsis are crucial to cut off its major medical consequences: lifelong morbidities, neurodevelopmental disabilities, and a high number of neonatal mortalities. Aim of the Work: This study is aimed at determining the diagnostic and prognostic performance of CD11b as a sepsis biomarker for detecting neonatal sepsis at early stages compared to nCD64 and the other conventional sepsis parameters. Methods: Two hundred eleven neonates were enrolled from three Egyptian neonatal ICUs (NICUs), and they were classified into two main groups: the control group (n = 101) and the sepsis group (n = 110). Enrolled neonates were subjected to full sepsis screening, including complete blood count (CBC), C-reactive protein (CRP), blood cultures, and flow cytometry analysis for both CD64 and CD11b on the neutrophil surface (results represented as a percentage (percent) and mean fluorescent intensity (MFI) units for either biomarker). Results: nCD64% (median = 44.15%) was significantly enhanced in the sepsis group compared to the controls (median = 25%), achieving 90.8% specificity, 92.8% sensitivity, and AUC = 0.894, respectively. CD64 MFI and CD11b MFI could differentiate between sepsis and control groups but with low undesirable diagnostic performance (sensitivity: 72.5% and 59.1%; specificity: 54.4% and 69.4%; AUC: 0.634 and 0.144, respectively). CD11b% could not discriminate between sepsis and control neonates (sensitivity and specificity of 31.8% and 73.6%, respectively) with an AUC of 0.405. hs-CRP had moderate diagnostic performance, achieving sensitivity and specificity of 69% and 78.15%, respectively, and AUC = 0.586. ROC analysis showed that combined hs-CRP and CD64% results had the highest sensitivity and specificity in the current study, being 93.9% and 97.2%, with AUC = 0.938, respectively. Conclusion: CD64%, CD64 MFI, CD11b MFI, and hs-CRP are increased in neonates with sepsis comparable to the controls. CD64% has a superior diagnostic performance comparable to nCD11b and hs-CRP. Combined nCD64 with hs-CRP measurement can provide rapid and accurate diagnostic modality for sepsis diagnosis in correlation with the patient's clinical condition and context with the results of other hematological indices; neutrophil CD64 can be routinely applicable in NICUs for better sepsis management. It is statistically evident that nCD11b is less ideal compared to nCD64 as a diagnostic, prognostic, or monitoring sepsis marker.
