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Updated: May 13, 2025

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

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Published on: July 2, 2018

High-Pressure Effects on Gelatin Sol-Gel Transition.

Nikolaos A Burger^1,2, Gerhard Meier³, Dimitris Vlassopoulos^1,2

¹Foundation for Research & Technology Hellas (FORTH), Institute for Electronic Structure & Laser, Heraklion 70013, Greece.

Industrial & Engineering Chemistry Research

|April 14, 2025

Summary

This summary is machine-generated.

High hydrostatic pressure accelerates gelatin

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Area of Science:

Biophysics
Materials Science
Rheology

Background:

Gelatin's sol-gel transition is crucial for its applications.
Understanding pressure effects on gelation is key for novel material processing.
High hydrostatic pressure (HHP) is a non-thermal processing technique.

Purpose of the Study:

To investigate the impact of high hydrostatic pressure on the sol-gel transition of gelatin.
To quantify pressure-induced changes in gelation kinetics and thermodynamics.
To elucidate the role of pressure in gelatin helix formation and stability.

Main Methods:

Dynamic Light Scattering (DLS) for monitoring particle size and dynamics.
DLS-based passive microrheology to assess viscoelastic properties.
Isothermal and reverse quenching experiments under varying hydrostatic pressures.

Main Results:

Critical gelation time (t_c) decreased with increasing pressure.
Critical temperature (T_c) increased with pressure (~0.04 K/MPa).
Pressure accelerated sol viscosity and gel elastic modulus evolution, indicating faster helix formation and stabilization.

Conclusions:

High hydrostatic pressure accelerates gelatin sol-gel transition kinetics.
Pressure stabilizes gelatin helices, enhancing gel strength and slowing melting.
Rheological changes under pressure directly correlate with helix formation processes.