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Related Experiment Video

Updated: May 13, 2025

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Modelling Skeletal Muscle Ageing and Repair In Vitro.

Janelle Tarum1, Hans Degens2,3, Mark D Turner4

  • 1Department of Sport Science, Sport Health and Performance Enhancement Research Centre (SHAPE), School of Science and Technology, Nottingham Trent University, Nottingham, UK.

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Ageing impairs skeletal muscle regeneration by affecting satellite cells and myogenesis. This study reveals molecular pathways involved in reduced muscle repair in older individuals, paving the way for new therapies.

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Area of Science:

  • Muscle biology and regenerative medicine
  • Gerontology and cellular ageing research

Background:

  • Skeletal muscle regeneration declines with age, primarily due to impaired satellite cell function and reduced myogenic capacity.
  • Understanding the molecular mechanisms behind age-related muscle regeneration deficits is crucial for developing effective therapeutic interventions.

Purpose of the Study:

  • To investigate the molecular pathways associated with reduced myogenesis and impaired muscle regeneration in aged versus young muscle cells.
  • To establish a high-throughput in vitro model for assessing muscle regeneration capacity across different age groups.

Main Methods:

  • Utilized a high-throughput in vitro model to assess muscle regeneration in chemically injured C2C12 and human myoblast cultures (young vs. aged).
  • Employed RNA-sequencing to analyze differential gene expression and identify enriched KEGG pathways and Gene Ontology (GO) processes.

Main Results:

  • Aged muscle cells exhibited significantly reduced regeneration capacity compared to young cells, showing attenuated recovery in myotube size and myogenic fusion index.
  • RNA-sequencing revealed significant enrichment of PI3K-Akt signaling pathways and downregulation of processes related to muscle development, differentiation, and contraction in aged cells.

Conclusions:

  • In vitro muscle repair is demonstrably impaired in aged compared to young muscle cells.
  • The study provides a framework for further research into the molecular factors contributing to age-related decline in muscle regeneration.