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Updated: Jul 17, 2026

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Published on: July 18, 2011
Autophagy-deficient budding yeast cells are sensitive to freeze-thaw stress
Maria James1,2, Grace K Klain1,3, Stacey O Brito1
1Department of Biological Sciences, Goucher College, Towson, Maryland, United States.
Abstract:
Autophagy enables eukaryotes to recycle damaged and unneeded materials to ensure survival in times of stress such as starvation. However, the full range of cellular stress responses that activate and require autophagy remains unknown. This study has compared the survival of wild type, atg1Δ, and atg5Δ budding yeast cells following freeze-thaw stress. The results indicate that cells deficient in autophagy exhibit enhanced sensitivity to freeze-thaw stress.
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