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Histopathological evaluation of viscoelastic POSS administered subcutaneously using a rat model
Hamed Benghuzzi1, Michelle Tucci2, Drew Hildebrandt2
1Jackson State University, 1400 John R. Lynch St, Jackson, MS 39217, USA.
Toxicology Reports
|April 14, 2025
Summary
Polyhedral oligomeric silsesquioxanes (POSS) gels show minimal long-term tissue reaction in rats over 180 days. These non-toxic materials form a connective tissue capsule, preventing migration and inflammation, making them suitable for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Polyhedral oligomeric silsesquioxanes (POSS) are cage-like silicon-oxygen nanostructures.
- POSS can be incorporated into polymer matrices or used as cross-linkers.
- POSS exhibits non-toxicity, improved mechanical properties, and biodegradation resistance, relevant for tissue engineering.
Purpose of the Study:
- To evaluate the long-term tissue response to Polyhedral oligomeric silsesquioxanes (POSS) gel injections in a rat model.
- To compare the tissue reaction to POSS gel versus air injections over 180 days.
Main Methods:
- Adult male Sprague Dawley rats were used.
- Rats received multiple injections of POSS gel (UVPOSS:POSS or POSS SO1455) or air (control).
- Tissues were harvested at various time points (7, 14, 21, 90, and 180 days) for analysis.
Main Results:
- POSS vesicles remained pliable for 180 days.
- Fluorescently labeled POSS showed no systemic mobility over 4 weeks.
- POSS induced a foreign body response with low-grade inflammation, forming a dense capsule that prevented migration.
Conclusions:
- Polyhedral oligomeric silsesquioxanes (POSS) are encapsulated by connective tissue.
- No long-term inflammatory cells or granuloma formation were observed up to 180 days.
- POSS demonstrates a favorable tissue response for potential biomedical applications.
