Colony morphotype variation in Burkholderia: implications for success of applications and therapeutics

The Burkholderia genus includes both environmental and pathogenic isolates known for their phenotypic plasticity and adaptability. Burkholderia spp. are intrinsically resistant to many antibiotics, often requiring prolonged therapies during infection. A key feature of Burkholderia spp. is colony morphotype variation (CMV), which allows for rapid adaptation to environmental changes and influences virulence, antibiotic resistance, and pathogenicity by impacting the expression of key virulence factors such as lipopolysaccharides, extracellular DNA, efflux pumps, and flagella. While alternative treatments, such as vaccines and phage therapies, hold promise, CMV has the potential to undermine their efficacy by modifying essential therapeutic targets. Despite its importance, the prevalence and underlying mechanisms of CMV remain poorly understood, leaving critical gaps in our knowledge that may hinder the development of sustainable solutions for managing Burkholderia infections. Addressing these gaps is crucial not only for improving infection management but also for enabling the safe reuse of Burkholderia in biotechnology, where their plant growth-promoting and bioremediation properties are highly valuable. Our goal is to raise awareness within the scientific community about the significance of CMV in Burkholderia, highlighting the urgent need to uncover the mechanisms driving CMV. A deeper understanding of CMV's role in virulence and resistance is essential to developing robust, long-term therapeutic strategies.