A Fluorescent Probe for Imaging and Treating S-Nitrosation Stress in OGD/R Cells

Area of Science:

• Biochemistry
• Cell Biology
• Medical Chemistry

Background:

• Protein S-nitrosation is a critical redox modification regulating protein function and signaling.
• Dysregulated S-nitrosation is linked to pathological conditions like oxygen-glucose deprivation/reperfusion (OGD/R) injury.
• There is a need for theranostic tools to manage S-nitrosothiol (SNO) levels in diseases, especially OGD/R models.

Purpose of the Study:

• To develop a selective probe for monitoring and modulating SNO levels.
• To investigate the probe's efficacy in an OGD/R cellular model.
• To assess the probe's potential as a theranostic agent for ischemia-reperfusion diseases.

Main Methods:

• Synthesis and characterization of a selective SNO-reactive probe, P-EHC.
• Utilizing live-cell confocal imaging to detect SNO levels in OGD/R-treated SH-SY5Y cells.
• Evaluating the therapeutic effects of P-EHC on reactive oxygen species (ROS), nitric oxide (NOx) species, and cell viability.

Main Results:

• P-EHC exhibits turn-on fluorescence, high quantum yield, and good water solubility.
• Elevated SNO levels were observed in the OGD/R cellular model.
• P-EHC treatment reduced intracellular ROS, lowered total NOx species, and improved cell viability in the OGD/R model.
• P-EHC demonstrated macrophage migration inhibitory factor (MIF) inhibitory activity.

Conclusions:

• P-EHC is a versatile probe for monitoring SNO in biological systems.
• The probe shows promise as a theranostic agent for ischemia-reperfusion diseases.
• P-EHC's simplicity and effectiveness suggest broad applicability in various disease models and therapeutic strategies.