Intelligent Point-of-Care Biosensing Platform Based on Luminescent Nanoparticles and Microfluidic Biochip with...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Analytical Chemistry

Background:

• Point-of-care diagnostics are crucial for timely cancer monitoring and screening.
• Existing methods for tumor marker detection often lack the required sensitivity or convenience for widespread use.
• Development of advanced biosensing platforms is needed to address these limitations.

Purpose of the Study:

• To design and manufacture an intelligent biosensing platform for point-of-care tumor marker biodetection.
• To leverage quantum dots luminescence and microfluidic biochip technology for enhanced diagnostics.
• To develop a machine vision algorithm for intelligent signal processing and analysis.

Main Methods:

• Quantum dots with high photoluminescent performance were modified with specific antibodies.
• A sandwich structure immunoassay was employed on a microfluidic biochip.
• A machine vision algorithm was integrated for intelligent biosensing data interpretation.

Main Results:

• The developed biosensing platform demonstrated high sensitivity, with a limit of detection around 0.021 ng/mL.
• The platform exhibited excellent accessibility and integrability for practical applications.
• Testing with human artificial saliva samples showed superior detection capabilities compared to commercial rapid test strips.

Conclusions:

• The quantum dot-based microfluidic biochip with intelligent algorithm offers a promising solution for point-of-care tumor marker diagnostics.
• This advanced biosensing platform provides a sensitive, convenient, and integrated approach for cancer monitoring and screening.
• The technology holds significant potential for future applications in the point-of-care diagnostics field.