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Updated: Jun 22, 2026
Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB
Published on: August 6, 2011
Multiplex PCR for the Rapid Diagnosis of Myeloproliferative Neoplasms
Laura Semenuk1,2, Alissa Palazzolo3, Kristen McCambridge3
1Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada. Laura.Semenuk@queensu.ca.
Abstract:
Myeloproliferative neoplasms (MPN) develop as a disease of aging, due to the disruption of normal hematopoiesis, triggered by somatic mutations and a sustained inflammatory cycle. Driving variants within JAK2, MPL, and CALR are associated with various subtypes of MPN, with known targeted therapeutics and guided patient prognosis. Targeted clinical testing for the diagnosis of these precise driving variants within these key genes has an integral role for personalized medicine.
