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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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The Impact of miRNA Expression on Colon Cancer Severity, Invasiveness, and Localization.

Constantin-Dan Tâlvan1, Elena-Teodora Tâlvan1, Călin Ilie Mohor1

  • 1Faculty of Medicine, "Lucian Blaga" University of Sibiu, 550169 Sibiu, Romania.

Cancers
|April 14, 2025
PubMed
Summary

Specific microRNAs (miRNAs) like miR-101-3p, miR-106a-5p, and miR-326 are linked to colorectal cancer (CRC) severity and invasion. Their expression patterns may aid in early detection and targeted CRC therapies.

Keywords:
biomarkerscolon cancergradinginvasivenesslocalizationmiRNA

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Colorectal cancer (CRC) presents a significant global health challenge with increasing incidence and mortality rates.
  • MicroRNAs (miRNAs) are implicated in the complex processes of tumor development, invasion, and metastasis.
  • Understanding miRNA dysregulation is crucial for advancing CRC research and treatment strategies.

Purpose of the Study:

  • To investigate the expression levels of miR-101-3p, miR-106a-5p, and miR-326 in colorectal cancer (CRC) patients.
  • To determine the association between the expression of these specific miRNAs and clinicopathological features such as tumor grade and tissue invasion.
  • To explore the potential of these miRNAs as biomarkers for CRC progression.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) was employed to measure miRNA expression in a cohort of 40 CRC patients.
  • Statistical analyses, including regression modeling, were utilized to assess correlations between miRNA expression levels and tumor characteristics.
  • Clinicopathological data, including tumor grade and invasion patterns, were analyzed in conjunction with miRNA expression data.

Main Results:

  • A significant downregulation of miR-101-3p was observed in advanced-stage colorectal tumors.
  • miR-106a-5p expression levels showed a notable peak in Grade 2 CRC, suggesting a role in intermediate tumor progression.
  • miR-326 expression was found to be associated with organ metastasis, highlighting its potential involvement in metastatic spread.
  • Regression analyses revealed intricate interaction patterns among the studied miRNAs, indicative of their collective role in CRC pathogenesis.

Conclusions:

  • The expression profiles of miR-101-3p, miR-106a-5p, and miR-326 are demonstrably associated with the severity and invasiveness of colorectal cancer.
  • These miRNAs hold promise as potential biomarkers for the early detection of CRC and the development of targeted therapeutic interventions.
  • Further extensive research involving larger patient cohorts is warranted to validate these findings and fully elucidate their clinical applicability in managing colorectal cancer.