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The bactericidal activity of aqueous disinfectants applied on living tissues
Abstract:
Thirteen antiseptic aqueous solutions intended for the disinfection of living tissues were compared in regard to their microbicidal effectiveness towards. Staphylococcus aureus and Pseudomonas aeruginosa. Six antiseptics, which contain boric acid, eosine, hydrogen peroxide or an organic mercury compound as the active substance, did not fulfil the requirements of the preliminary in vitro test. The seven other preparations were examined in a practical test, in which bactericidal activity was assessed on artificially contaminated intact skin after exposures of 15 s and 60 s. The most active solution appeared to be 0.5% tosylchloramide sodium, followed by 0.05% chlorhexidine with 0.5% cetrimide. The other preparations, namely 0.05% chlorhexidine without cetrimide, 0.245% chloroxylenol, 0.04% clorofene, 10% povidone-iodine and 0.2% tosylchloramide sodium, were less active in this practical test.
Insights
This study compared 13 antiseptic solutions for effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa. The most effective antiseptic for disinfecting living tissues was 0.5% tosylchloramide sodium.
Area of Science:
- Microbiology
- Antiseptics and Disinfectants
Background:
- Antiseptic solutions are crucial for preventing infections by eliminating microorganisms from living tissues.
- Assessing the efficacy of various antiseptic formulations against common pathogens like Staphylococcus aureus and Pseudomonas aeruginosa is vital for clinical practice.
Purpose of the Study:
- To evaluate and compare the microbicidal effectiveness of thirteen different antiseptic aqueous solutions.
- To identify the most potent antiseptic for skin disinfection against specific bacterial strains.
Main Methods:
- An in vitro preliminary test was conducted to screen antiseptic solutions.
- A practical test assessed bactericidal activity on artificially contaminated intact skin following 15-second and 60-second exposures.
Main Results:
- Six antiseptics containing boric acid, eosine, hydrogen peroxide, or organic mercury compounds failed the initial in vitro test.
- 0.5% tosylchloramide sodium demonstrated the highest bactericidal activity.
- 0.05% chlorhexidine with 0.5% cetrimide was the second most effective antiseptic tested.
Conclusions:
- Tosylchloramide sodium and chlorhexidine with cetrimide are highly effective antiseptics for skin disinfection.
- Several common antiseptic ingredients (boric acid, eosine, hydrogen peroxide, organic mercury) were found ineffective in this study.
- The study provides valuable data for selecting appropriate antiseptic agents in healthcare settings.