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Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro.
Muhanna Alshehri1, Saeed Alqahtani2, Pranitha Murali3
1Oral Sciences Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow; Glasgow Biofilm Research Network.
Journal of Visualized Experiments : Jove
|April 14, 2025
Summary
Commercially available epithelial models offer reproducible platforms for studying host-pathogen interactions in vitro. These models enable assessment of microbial invasion and host response, aiding antimicrobial testing.
Area of Science:
- Microbiology and Immunology
- Infectious Diseases
- Biotechnology
Background:
- Replicating host-pathogen interactions at epithelial barriers in vitro is challenging.
- Traditional 2D cell cultures lack in vivo relevance.
- Commercially available tissue models are often underutilized due to cost and practicality concerns.
Purpose of the Study:
- To report the utility of epithelial models for investigating host-pathogen interactions.
- To demonstrate their application across diverse ecological niches (oral, skin, vaginal).
- To showcase their use in evaluating antimicrobial efficacy in vitro.
Main Methods:
- Utilized commercially available epithelial models for co-culture experiments.
- Assessed microbial adherence, invasion, and host response (transcriptional/protein levels).
- Employed quantitative PCR (qPCR) and ELISA for host gene expression and protein detection.
Main Results:
- Epithelial models successfully supported co-culture with planktonic cells and biofilms.
- Demonstrated assessment of microbial interactions and host inflammatory responses.
- Facilitated evaluation of antimicrobial activity within a complex microenvironment.
Conclusions:
- Epithelial models provide a reproducible and versatile platform for in vitro host-pathogen studies.
- These models are valuable for preclinical investigations of infectious diseases.
- They offer a more accurate representation of host-pathogen dynamics compared to 2D cultures.
