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Updated: May 6, 2026
High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Published on: November 28, 2016
Establishment of a microfluidic and RPA-based platform for rapid multi-sample detection of Mycoplasma pneumoniae
Ling Zhang1, Cheng-Di Sun1, Jiao Luo1
1Department of Laboratory Medicine, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Background:
Mycoplasma pneumoniae (M. pneumoniae) is a major respiratory pathogen causing atypical community-acquired pneumonia. Early and rapid diagnosis and timely detection of pathogens are crucial. RPA is an emerging PCR technology that allows amplification at room temperature compared to conventional PCR technology, while combining the advantages of rapid detection and high specificity of PCR technology, enabling rapid and accurate detection of pathogens.
Methods And Results:
In this study, we established a platform for detection of M. pneumoniae based on microfluidic and RPA technologies. The RPA technology is performed in 15 to 20 minutes, and only M. pneumoniae is significantly amplified at a sensitivity of 10 copies/μL; microfluidic technology provides lean instrumentation and a convenient interactive system. Three groups negative and positive QCs and 18 clinical samples can be tested in a single run.
Significance:
In conclusion, we have established a microfluidic molecular assay operating system based on RPA technology, with the advantages of being simple, rapid, accurate and economical, and providing a new tool for laboratory detection of M. pneumoniae.
