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A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

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Published on: July 22, 2017

Needle in a Haystack: Culturing Plant-Beneficial Helotiales Lineages From Plant Roots.

Pauline Bruyant¹, Jeanne Doré¹, Laurent Vallon¹

¹Laboratoire d'Ecologie Microbienne LEM, CNRS UMR5557, INRAE UMR1418, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, Villeurbanne, France.

Environmental Microbiology

|April 14, 2025

Summary

This summary is machine-generated.

Alpine non-mycorrhizal plants host beneficial Helotiales fungi in their roots. Cultured isolates show potential for promoting plant growth, offering a resource for studying these fungi.

Keywords:

Brassicaceae Caryophyllaceae Cyperaceae Helotiales fungal collection non‐mycorrhizal plants root endophytes

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Published on: March 1, 2022

Area of Science:

Plant-microbe interactions
Fungal ecology
Alpine botany

Background:

Root-associated Helotiales fungi benefit plants, especially non-mycorrhizal hosts.
The ecology and cultivation of these beneficial fungi remain poorly understood.

Purpose of the Study:

To isolate and identify plant-beneficial Helotiales fungi from alpine non-mycorrhizal plants.
To assess the culturability and diversity of root endophytic fungi.
To compare culture-based isolation with fungal metabarcoding.

Main Methods:

Sampling of six wild non-mycorrhizal plant species from alpine soils.
Mass isolation of root endophytic fungi using a tailored culture-based approach.
Fungal ITS2 metabarcoding for community analysis.
Phylogenetic analysis of isolated Helotiales strains.

Main Results:

602 root endophytes were isolated, with 53% identified as Helotiales.
Metabarcoding confirmed Helotiales dominance in root fungal communities (43% of detected taxa).
Isolated Helotiales belonged to three clades, related to plant growth-promoting strains, some showing P solubilisation.

Conclusions:

Alpine non-mycorrhizal plant roots harbor diverse, plant-beneficial Helotiales fungi.
The obtained isolates are a valuable resource for exploring fungal plant-beneficial mechanisms and ecological roles.
Culture-based methods can effectively recover key fungal lineages from plant roots.