CMSS1: A RNA binding protein with pivotal roles in non-small cell lung cancer progression and prognosis
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View abstract on PubMed
Summary
This summary is machine-generated.The Cms1 ribosomal small subunit homolog (CMSS1) is upregulated in non-small cell lung cancer (NSCLC), correlating with poor prognosis. Downregulating CMSS1 inhibits tumor growth, suggesting it as a therapeutic target for NSCLC.
Area Of Science
- Oncology
- Molecular Biology
- Immunology
Background
- The Cms1 ribosomal small subunit homolog (CMSS1), an RNA-binding protein (RBP), is implicated in tumor development.
- The prognostic and immunological significance of CMSS1 in non-small cell lung cancer (NSCLC) is not well understood.
Purpose Of The Study
- To investigate the prognostic and immunological role of CMSS1 in NSCLC.
- To evaluate CMSS1 as a potential therapeutic target and prognostic biomarker for NSCLC.
Main Methods
- Utilized The Cancer Genome Atlas (TCGA) database to identify differentially expressed RBP genes.
- Selected CMSS1 as a hub gene via univariate Cox regression and Kaplan-Meier analyses.
- Assessed prognostic capacity using time-dependent ROC curves and multivariate Cox regression.
- Analyzed tumor-infiltrating immune cells with the ImmuCellAI algorithm.
- Performed loss-of-function assays to determine CMSS1's functional role in NSCLC cells.
Main Results
- CMSS1 was significantly upregulated in NSCLC tumors, with higher expression linked to poorer patient prognosis.
- CMSS1 expression correlated negatively with CD4 T cells and positively with macrophages and Tregs.
- Loss-of-function assays demonstrated that CMSS1 downregulation inhibited NSCLC cell viability, migration, and invasion.
- RT-qPCR and western blot confirmed elevated CMSS1 mRNA and protein levels in NSCLC cell lines.
Conclusions
- CMSS1 may function as a prognostic indicator and a therapeutic target in NSCLC.
- Findings offer potential guidance for precision therapy and prognosis prediction in NSCLC patients.
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