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The Potential Biological Roles and Clinical Significance of Anaphase-Promoting Complex Subunit 1 in Colorectal Cancer
Yi Chen1, Yu-Xing Tang1, Da-Tong Zeng2
1Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, P.R. China.
Cancer Control : Journal of the Moffitt Cancer Center
|April 15, 2025
Summary
Anaphase-promoting complex subunit 1 (ANAPC1) is overexpressed in colorectal cancer (CRC) and drives tumor growth. Lower ANAPC1 expression may predict better response to immune checkpoint inhibitors in CRC patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Anaphase-promoting complex subunit 1 (ANAPC1) is a key regulator of mitosis and implicated in tumorigenesis.
- A comprehensive understanding of ANAPC1's role in colorectal cancer (CRC) is currently lacking.
Purpose of the Study:
- To investigate the expression patterns, biological functions, and clinical significance of ANAPC1 in colorectal cancer.
- To explore ANAPC1's potential as a biomarker for CRC progression and treatment response.
Main Methods:
- Integrated analysis of large-scale mRNA expression data (2329 samples) and single-cell RNA sequencing.
- Immunohistochemistry validation on 416 CRC tissue samples.
- In vitro experiments including RT-PCR, Western blot, CCK-8, wound healing, cell cycle, and apoptosis assays.
Main Results:
- ANAPC1 mRNA and protein were significantly overexpressed in CRC tissues and malignant cells.
- ANAPC1 knockdown reduced CRC cell proliferation and migration while increasing apoptosis and altering cell cycle.
- Low ANAPC1 expression correlated with improved patient response to immune checkpoint inhibitors, including PD-1 inhibitors.
Conclusions:
- ANAPC1 overexpression promotes CRC development through pathways involving DNA replication, chromosome segregation, and ribosome biogenesis.
- ANAPC1 serves as a potential prognostic biomarker and may predict immunotherapy response in colorectal cancer.
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