Related Experiment Video

Updated: May 13, 2025

Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.5K

Transcription factor occupancy limits DNA methylation and determines ICAM1 expression in breast cancer

Mingcang Chen1,2, Ying Zhou3, Zhengwei Fu1

  • 1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.

Acta Biochimica Et Biophysica Sinica
|April 15, 2025
PubMed

Insights

Transcription factor (TF) binding reduces DNA methylation at the ICAM1 promoter, increasing ICAM1 expression in breast cancer. This epigenetic regulation is crucial for understanding TF roles in cancer gene expression.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Cancer Biology

Background:

  • The interplay between transcription factor (TF) binding and DNA methylation is vital for gene expression regulation.
  • The specific role of this interaction in ICAM1 (Intercellular Adhesion Molecule 1) expression within breast cancer remains unclear.

Purpose of the Study:

  • To investigate the relationship between TF binding, DNA methylation, and ICAM1 expression in breast cancer.
  • To elucidate the molecular mechanisms underlying ICAM1 regulation in breast cancer.

Main Methods:

  • Correlation analysis of CpG methylation, ICAM1 expression, and DNMT/TET3 levels in breast cancer.
  • Investigating the effect of TF binding on ICAM1 promoter methylation.
  • Analyzing the recruitment of TET3 by transcription factors (RELA, STATs) to the ICAM1 promoter.

Main Results:

  • CpG methylation in the ICAM1 promoter negatively correlates with ICAM1 expression.
  • ICAM1 expression positively correlates with DNA methyltransferase (DNMT) and TET3 expression.
  • TF binding reduces ICAM1 promoter methylation and enhances ICAM1 transcription.
  • RELA and STATs recruit TET3 to inhibit DNMT-mediated methylation, maintaining promoter hypomethylation.

Conclusions:

  • TF occupancy modulates DNA methylation at the ICAM1 promoter, thereby influencing ICAM1 expression in breast cancer.
  • Epigenetic mechanisms involving TF-TET3-DNMT interactions are key regulators of ICAM1 in breast cancer.
Keywords:
DNA methylationbreast cancercompetitionintercellular adhesion molecule 1transcription factor binding

More Related Videos

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.6K
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors06:07

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

2.5K

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.4K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
32.9K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.2K
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.1K