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Related Experiment Videos

Effect of diltiazem on in vitro rabbit bladder function.

S B Malkowicz, A J Wein, K Brendler

    Pharmacology
    |January 1, 1985
    PubMed
    Summary

    Extracellular calcium is crucial for rabbit urinary bladder emptying. Diltiazem, a calcium antagonist, inhibits bladder function, but this effect can be overcome by increasing calcium levels.

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    Area of Science:

    • Urology
    • Pharmacology
    • Physiology

    Background:

    • Extracellular calcium plays a role in smooth muscle contraction.
    • Urinary bladder function relies on complex signaling pathways involving calcium.
    • Calcium antagonists are used to treat various conditions, but their effect on bladder function requires further investigation.

    Purpose of the Study:

    • To investigate the relationship between extracellular calcium and urinary bladder function.
    • To determine the effect of the calcium antagonist diltiazem on in vitro rabbit urinary bladder emptying.
    • To explore the potential therapeutic role of diltiazem in urinary bladder dysfunction.

    Main Methods:

    • Studied the in vitro whole rabbit urinary bladder.
    • Administered the calcium antagonist diltiazem at various concentrations.
    • Investigated the effect of different extracellular calcium concentrations on bladder emptying stimulated by bethanechol and KCl.

    Main Results:

    • A specific extracellular calcium concentration (4.5 X 10(-4) M) was required for near-complete cholinergic-mediated emptying.
    • Diltiazem's inhibition of bladder function was competitively antagonized by increased extracellular calcium.
    • KCl-mediated emptying was more sensitive to diltiazem inhibition than bethanechol-stimulated emptying, with higher calcium concentrations not fully reversing the inhibition.

    Conclusions:

    • Diltiazem inhibits urinary bladder function, potentially by affecting both extracellular calcium influx and intracellular calcium stores.
    • The findings suggest diltiazem may be a potential therapeutic agent for certain urinary bladder dysfunctions.
    • Further research is warranted to explore diltiazem's therapeutic potential in urological conditions.

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