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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: May 13, 2025

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

Ion-Selective Channels in K562 Cells: A Patch-Clamp Analysis.

Jürgen Rettinger, Wolfgang Schwarz

Journal of Basic and Clinical Physiology and Pharmacology

|April 15, 2025

Summary

This summary is machine-generated.

Patch-clamp electrophysiology revealed four ion channels in K562 cells, including sodium, calcium-activated, anion-selective, and palytoxin-induced channels, offering insights into cellular ion transport mechanisms.

Keywords:

anion channel anion exchange cation channel erythroleucemia palytoxin patch clamp sodium pump

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Published on: May 22, 2017

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

Related Experiment Videos

Last Updated: May 13, 2025

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Published on: December 9, 2022

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Published on: May 22, 2017

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Published on: October 1, 2010

Area of Science:

Cellular Electrophysiology
Ion Channel Biology
Human Erythroleukemia K562 Cells

Background:

Understanding ion channel function is crucial for cellular physiology.
K562 cells are a relevant model for studying human blood cell ion transport.

Purpose of the Study:

To characterize ion-selective channels in human erythroleukemia K562 cells using patch-clamp techniques.
To identify the properties and potential molecular identities of these channels.

Main Methods:

Patch-clamp electrophysiology in cell-attached configuration.
Analysis of single-channel conductance, selectivity, and gating kinetics.
Application of specific channel modulators (TTX, Ca2+, TEA, H2DIDS, palytoxin).

Main Results:

Identified an 8 pS TTX-sensitive Na+ channel.
Characterized a 19 pS cation channel activated by intracellular Ca2+ and inhibited by TEA.
Described a rectifying anion channel with specific selectivity and H2DIDS sensitivity.
Induced an 8 pS Na+/K+ selective channel with palytoxin, potentially involving the Na+/K+-pump.

Conclusions:

K562 cells express diverse ion-selective channels, including Na+, Ca2+-activated cation, and anion channels.
Palytoxin can induce a Na+/K+ selective channel, possibly related to the Na+/K+-pump.
The anion channel may represent a conformation of an anion exchanger.