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Overview Of Cell Separation And Isolation01:20

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Updated: May 13, 2025

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
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Affinity-Based Isolation and One-Pot Analysis of Extracellular Vesicles from Biofluids Using Phase Separated

Francesca Torrini1, Roberto Frigerio2, Jonathan Garlipp1

  • 1Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich, Zurich, 8093, Switzerland.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|April 15, 2025
PubMed
Summary
This summary is machine-generated.

Zwitterionic coacervates enable selective isolation of extracellular vesicles (EVs) from biofluids. This method streamlines EV analysis for diagnostics and drug delivery applications.

Keywords:
biofluidsdiagnostic analysisextracellular vesiclesisolationliquid biopsyseparationzwitterionic coacervates

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Area of Science:

  • Biotechnology
  • Materials Science
  • Biochemistry

Background:

  • Extracellular vesicles (EVs) are crucial for diagnostics and therapeutics.
  • Isolating EVs from complex biofluids like urine is challenging due to their heterogeneity.
  • Current isolation methods struggle with efficiency, selectivity, and removing contaminants like lipoproteins.

Purpose of the Study:

  • To develop a novel method for efficient and selective isolation of EVs.
  • To demonstrate the utility of functionalized zwitterionic (ZW) coacervates for EV isolation and analysis.
  • To overcome limitations of conventional EV separation techniques.

Main Methods:

  • Development of phase-separated zwitterionic (ZW) coacervates.
  • Functionalization of ZW coacervates with a membrane-sensing peptide for affinity-based EV capture.
  • Application of the method to isolate EVs from urine samples.
  • Analysis of isolated EVs using flow cytometry.

Main Results:

  • ZW coacervates provide selective EV isolation across various pH and ionic strengths.
  • The method achieves high yields of structurally intact EVs from urine.
  • Efficient separation of EVs from lipoproteins was demonstrated.
  • A one-pot assay for EV biomarker profiling was successfully implemented, streamlining workflows.

Conclusions:

  • Functionalized ZW coacervates offer an effective strategy for selective EV isolation and direct analysis.
  • This approach advances EV-based liquid biopsy and drug delivery applications.
  • The method paves the way for improved diagnostics and large-scale EV manufacturing.