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Virome capture sequencing for comprehensive HPV genotyping in cervical samples
Thanayod Sasivimolrattana1,2, Sasiprapa Liewchalermwong2, Wasun Chantratita3
1Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand.
Science Progress
|April 15, 2025
Summary
Next-generation sequencing (NGS) using VirCapSeq offers superior human papillomavirus (HPV) genotyping compared to traditional reverse blot hybridization assay (REBA), detecting all HPV DNA and identifying mixed infections.
Area of Science:
- Genomics
- Molecular Diagnostics
- Oncology
Background:
- Cervical cancer screening relies on accurate human papillomavirus (HPV) detection and genotyping.
- Conventional methods like reverse blot hybridization assay (REBA) have limitations in detecting diverse HPV genotypes and mixed infections.
Purpose of the Study:
- To compare the performance of VirCapSeq, a next-generation sequencing (NGS) approach, with REBA for HPV genotyping in cervical specimens.
- To evaluate the diagnostic capabilities of NGS for comprehensive HPV detection and characterization.
Main Methods:
- Bioinformatic analysis of HPV genotypes from 35 cervical specimens using VirCapSeq and REBA methods.
- Mapping sequence data to the PaVE database for HPV genotyping.
- Stratification of specimens by histology into cervical intraepithelial neoplasia (CIN) grades.
Main Results:
- VirCapSeq (NGS) detected HPV DNA in 100% of samples, compared to 85.71% for REBA.
- NGS identified a broader range of HPV genotypes and detected mixed infections in all samples.
- HPV16 and HPV52 were the most prevalent genotypes identified by NGS.
Conclusions:
- NGS technologies, specifically VirCapSeq, demonstrate enhanced sensitivity and diagnostic capabilities for comprehensive HPV genotyping.
- Discrepancies between methods are linked to HPV genome abundance, coverage, and diversity.
- NGS holds significant potential for advancing cervical cancer screening and epidemiological studies.
