Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.0K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.0K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

9.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
9.6K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

8.4K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
8.4K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

153
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
153
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

12.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to 'Human-relevant exposure to mixtures of rare earth elements disrupts T3 homeostasis through impaired synthesis and conversion in human Thyrocytes' [Toxicology and Applied Pharmacology volume 514 (2026) 117929].

Toxicology and applied pharmacology·2026
Same author

AFM-based nanomechanical evaluation of human umbilical cord mesenchymal stem cells for alcohol-associated liver injury.

Analytical methods : advancing methods and applications·2026
Same author

Extrinsic n‑Doping of a Double B←N Bridged Bipyridine-Based Polymer Containing Oligoethylene Glycol Side Chains.

Polymer science & technology (Washington, D.C.)·2026
Same author

Parameter-Free Attention Super-Resolution Network for Accelerated AFM Biological Imaging.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Pathogen-Mimicking Nanovaccine Induces Potent Humoral and Cellular Immunity against <i>Brucella</i>.

ACS applied materials & interfaces·2026
Same author

T-cell mechanobiology: How molecular forces shape immune function.

The Journal of cell biology·2026

Related Experiment Video

Updated: May 13, 2025

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.2K

Light-Induced Electrode Scanning Microscopy.

Fengyan Hou1,2,3, Huanzhou Yang1,2,3, Jianjun Dong1,2,3

  • 1International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China.

Analytical Chemistry
|April 15, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel light-induced electrode scanning microscopy, a non-invasive tool for detecting cellular electrical properties. This technology scans living cells, offering a new approach to electrophysiology.

More Related Videos

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
00:07

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

7.9K
Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM
08:31

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Published on: February 10, 2021

6.7K

Related Experiment Videos

Last Updated: May 13, 2025

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

1.2K
Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
00:07

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

7.9K
Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM
08:31

Probing Surface Electrochemical Activity of Nanomaterials using a Hybrid Atomic Force Microscope-Scanning Electrochemical Microscope AFM-SECM

Published on: February 10, 2021

6.7K

Area of Science:

  • Biomedical Engineering
  • Cellular Electrophysiology
  • Microscopy

Background:

  • Conventional patch clamp and microelectrode array techniques for detecting cellular electrical properties are invasive or limited to fixed positions.
  • There is a need for advanced, non-invasive methods to study cell electrophysiology across larger areas.

Purpose of the Study:

  • To develop and present a novel light-induced electrode scanning microscopy system.
  • To enable non-invasive, large-area detection of electrical signals from single living cells in culture.

Main Methods:

  • Utilizing light-induced electrode scanning (LAPS) and optically induced dielectrophoresis principles.
  • Employing a photosensitive chip where projected light patterns form dynamic electrodes.
  • Implementing scanning by moving the light pattern or the chip for comprehensive area coverage.

Main Results:

  • Demonstrated a "radar-like" scanning capability across a whole area of living cells.
  • Successfully detected electrical signals from single cells and determined cell localizations.
  • Established a new method for measuring electrical characteristics of biological cells.

Conclusions:

  • The developed light-induced electrode scanning microscopy is a novel tool for detecting cell electrical properties.
  • This technology has the potential to become the next generation of electrophysiological detection.
  • Offers a non-invasive, high-resolution alternative to existing electrophysiological methods.