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Updated: May 13, 2025
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
Published on: February 20, 2019
LINC00426 promotes the progression of atherosclerosis by regulating miR-873-5p/SRRM2 axis
1Department of Cardiology, Zhejiang Provincial People's Hospital Bijie Hospital, Bijie 551700, China.
Background:
Atherosclerosis (AS) is a disease that occurs in the great arteries and is the main cause of cardiovascular disease and death.
Objective:
To investigate the clinical significance of LINC00426 in AS and to investigate that LINC00426 regulates PDGF-BB-induced proliferation, migration, invasion and inflammatory response of vascular smooth muscle cells (VSMCs) by modulating miR-873-5p/SRRM2 axis.
Methods:
The expression of LINC00426 was detected using RT-qPCR. The diagnostic role of LINC00426 in AS was analyzed with ROC curves. CCK-8 assay was used to measure cell proliferation, and transwell assay was used to measure cell migration and invasion ability. The targeted binding relationship between LINC00426 and miR-873-5p, miR-873-5p and SRRM2 was detected using dual-luciferase reporter gene assay. The concentration of proinflammatory factors was detected by using ELISA kit.
Result:
The expression of LINC00426 was increased in patients with AS, and LINC00426 had a diagnostic role in AS. In addition, LINC00426 regulated PDGF-BB-induced proliferation, migration, invasion, and inflammation of VSMCs by regulating miR-873-5p/SRRM2 axis.
Conclusion:
LINC00426 may function as a biomarker for the diagnosis and treatment of AS.
