A novel partitivirus with four dsRNA segments causing no obvious symptoms in Aspergillus flavus

Aspergillus flavus partitivirus 2 (AfPV2) isolate XC-8 from the fungus Aspergillus flavus strain XC-8 was sequenced and analyzed. AfPV2 contains four segments, dsRNA1 to 4. dsRNA1 is 1907 bp in length with an open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) of 565 amino acids (aa). dsRNA2 is 1936 bp in length with an ORF encoding a putative capsid protein (CP) of 508 aa. dsRNA3 is 1799 bp in length with an ORF encoding a hypothetical protein of 482 aa. dsRNA4 is 1650 bp in length with an ORF encoding a hypothetical protein of 400 aa. Phylogenetic analysis showed that AfPV2 is a member of the genus Alphapartitivirus of the family Partitiviridae. BLASTp analysis showed that AfPV2 isolate XC-8 belongs to the same species as AfPV2 isolate UniPR6, which only has two dsRNA segments (GenBank nos. MZ600060.1 and MZ600061.1). Infection by AfPV2 isolate XC-8 did not cause any obvious significant phenotypic changes in A. flavus.