Determination of reference intervals for common liver function tests among healthy adults

Reference intervals (RIs) are significant means for health evaluation, prognosis, diagnosis, and monitoring of adverse events. The RIs are affected by ethnicity, age, gender, geographic area, diet, socioeconomic, and physical situation. This study aimed to determine RIs of commonly used liver function tests (LFT) for healthy adults in Ibb City, middle of Yemen. A total of 390 participants aged between 18 and 70 were selected and administered a questionnaire. Blood specimens were assembled after an overnight fast, and the sera were separated for analysis of common LFT (DBIL, TBIL, ALB, TP, ALP, AST, and ALT) using Mindray BS-240 Automatic Clinical Chemistry Analyzer. The data were computed by GraphPad Prism 8.0.1. This study revealed that RIs for males and females of DBIL, TBIL, ALB, ALP, and ALT were significantly higher in males than females. Although RIs for TP and AST were higher in males than females, the difference was non-significance. Notably, most of the RIs in our study were different than those from other countries, either higher or lower. In conclusion, this study has established a panel of locally relevant RIs for commonly used LFT in adults, Ibb City, which may help interpret laboratory results for healthy adults and patients.