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Updated: May 13, 2025
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
A case report of bacterial canaliculitis caused by Ottowia massiliensis Sp.nov
Yuxuan Wu1, Hua Sun1, Min Kang1
1Beijing Tongren Hospital, Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Capital Medical University, Beijing, 100005, China.
Background:
Ottowia massiliensis sp.nov. is a slow-growing and Gram-stain-negative bacillus, first isolated from the stool sample of a healthy volunteer. Due to its slow growth and challenging cultivation, it is easily overlooked in clinical microbiology laboratories. This case report is the first case of its pathogenicity.
Case Presentation:
This case report describes a case of canaliculitis caused by Ottowia massiliensis sp.nov. The patient, an 80-year-old woman, presented with bilateral epiphora and discharge lasting for 4 years, which worsened with redness and swelling over the past week. Microbial culture of the lacrimal duct secretion after 72 h on Columbia blood agar showed small colonies. MALDI-TOF MS did not yield accurate results, but 16 S rDNA sequencing identified the bacterium as Ottowia massiliensis sp.nov. (LT960589.1). Antimicrobial sensitivity tests showed high activity against most ophthalmic drugs.
Conclusions:
In the case described here, we discuss the diagnosis and treatment strategies for eye infections caused by Ottowia massiliensis sp.nov., laying the foundation for improving clinical and laboratory awareness of this bacterium.
