Related Experiment Video
Updated: May 13, 2025
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Postoperative Ileus Murine Model
Published on: July 12, 2024
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Spontaneous ileitis and postsurgical murine models of enteric hyperoxaluria
Karim Jaber1, Nadim Zaidan1, Melody Ho1
1Division of Nephrology, Department of Medicine, NYU Langone Medical Center, New York, New York, United States.
Summary
New mouse models were developed to study enteric hyperoxaluria, a condition linked to kidney stones. These models simulate gastric bypass and Crohn's ileitis, aiding research into fat malabsorption and inflammation.
Area of Science:
- Gastroenterology
- Nephrology
- Animal Models
Background:
- Enteric hyperoxaluria (EH) is a risk factor for kidney stones, often caused by malabsorptive surgeries or inflammatory bowel diseases.
- Existing mouse models for studying EH are limited, hindering research into its mechanisms and potential treatments.
Purpose of the Study:
- To establish two novel and distinct mouse models for investigating enteric hyperoxaluria.
- One model simulates Roux-en-Y gastric bypass surgery, while the other mimics Crohn's ileitis.
Main Methods:
- Diet-induced obese mice underwent sham or bypass surgery, followed by a high-fat, oxalate-enriched diet.
- SAMP1/YitFc and AKR mice were fed high-fat diets supplemented with oxalate and reduced fat.
- Urine, blood, and fecal samples were analyzed for oxalate, creatinine, and lipids.
Main Results:
- Both mouse models exhibited hyperoxaluria and increased fecal fat, indicating fat malabsorption.
- Kidney injury was observed in affected mice.
- The study identified key roles for dietary oxalate, intestinal inflammation, and fat malabsorption in EH progression.
Conclusions:
- Two novel mouse models for enteric hyperoxaluria have been successfully established.
- These models demonstrate the impact of Roux-en-Y gastric bypass and ileitis on EH.
- The findings provide valuable tools for exploring EH mechanisms and developing therapeutic strategies.
