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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jun 19, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

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Optimizing Protocols for MicroRNA Profiling of Infant and Toddler Stool.

David A Armstrong, Shannon M Soucy, Meghan E Muse

    Biorxiv : the Preprint Server for Biology
    |April 16, 2025
    PubMed
    Summary
    This summary is machine-generated.

    High-quality RNA and similar microRNA profiles were obtained from infant stool using RNAlater or Zymo DNA/RNA Shield tubes with the Zymo BIOMICs kit. These findings support optimized protocols for using infant stool microRNAs as biomarkers for child development and disease.

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    Fecal micro RNA Isolation
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    Area of Science:

    • Biomarker discovery
    • Pediatric research
    • Molecular diagnostics

    Background:

    • MicroRNAs (miRNAs) show promise as biomarkers for child development and disease.
    • Infant and toddler stool is increasingly used for transcriptomic analysis.
    • Challenges include high RNase levels and abundant microbial RNA in stool specimens.

    Purpose of the Study:

    • To compare RNA preservation and extraction protocols for infant and toddler stool.
    • To evaluate commercial kits and preservation methods for RNA quality and miRNA profiling.
    • To establish optimized protocols for miRNA analysis in pediatric stool samples.

    Main Methods:

    • Phase 1: Compared three RNA extraction kits and four preservation methods using fragment analysis for RNA quality.
    • Phase 2: Utilized miRNA sequencing (miRNA-seq) to compare miRNA profiles from stool preserved in RNAlater versus Zymo DNA/RNA Shield tubes.
    • Bioinformatic analysis included classification of reads (human vs. microbial) and alignment to miRBase v22.1.

    Main Results:

    • The Zymo BIOMICs kit yielded the highest RNA Quality Number (RQN).
    • RNAlater and Zymo DNA/RNA Shield tubes provided comparable high-quality RNA yields.
    • No significant differences were observed in human miRNA profiles or detected miRNA counts between the two preservation methods.

    Conclusions:

    • Collecting infant stool in RNAlater or Zymo DNA/RNA Shield tubes, combined with the Zymo BIOMICs kit, yields high-quality RNA and consistent miRNA profiles.
    • Several detected miRNAs (e.g., miR-194a-3p, miR-200c-3p, miR-26a-5p) are implicated in gut homeostasis.
    • These findings provide a basis for standardized protocols in pediatric stool miRNA biomarker studies.