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Updated: Jun 15, 2026

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

Suite3D: Volumetric cell detection for two-photon microscopy.

Ali Haydaroğlu¹, Tinya Chang¹, Andrew Landau¹

¹University College London, London, UK.

Biorxiv : the Preprint Server for Biology

|April 16, 2025

Summary

This summary is machine-generated.

Suite3D is a new volumetric cell detection pipeline that enhances two-photon imaging analysis. It corrects for brain motion and improves neuron detection, offering a faster and more accurate solution for analyzing 3D neuronal activity.

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Last Updated: Jun 15, 2026

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

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Published on: January 19, 2011

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Published on: April 7, 2014

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices

Published on: March 26, 2018

Area of Science:

Neuroscience
Biophysics
Computational Biology

Background:

Two-photon imaging commonly acquires 3D neuronal activity data.
Current plane-by-plane processing limits accuracy due to duplicated cells, motion artifacts, and missed neurons.

Purpose of the Study:

Introduce Suite3D, a novel volumetric cell detection pipeline.
Address limitations of 2D processing for 3D two-photon microscopy data.

Main Methods:

Suite3D corrects for 3D brain motion, including axial and lateral movements.
Utilizes 3D correlation for improved signal-to-background ratio and neuron detectability.
Performs 3D segmentation to detect cells across multiple imaging planes.

Main Results:

Suite3D successfully detected cells across multiple imaging planes.
Improved cell detectability and signal quality compared to traditional methods.
Demonstrated >20x speed improvement over a prior volumetric pipeline.

Conclusions:

Suite3D provides a robust solution for analyzing volumetric two-photon microscopy data.
Enhances accuracy, efficiency, and cell detection in 3D neuronal imaging.
Overcomes limitations of plane-by-plane analysis in neuroscience research.