Related Experiment Video
Updated: May 13, 2025
Induction of Myocardial Infarction and Myocardial Ischemia-Reperfusion Injury in Mice
Published on: January 19, 2022
Effects of sevoflurane and isoflurane on acute myocardial infarction model establishment in mice
Jia-Nan Li1, Liu-Hao-Nan Zeng1, Lu Jin1
1Department of Anesthesiology, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, 410005, Hunan Province, China.
Abstract:
The selection of anesthetic drugs in the preparation of an acute myocardial infarction (AMI) model is very important. We specifically focus on various effects of sevoflurane and isoflurane in a murine AMI model, which have not been previously compared. Furthermore, we evaluated success of our AMI model using following methods: echocardiography, TTC staining, and PCR testing. The results show that compared to the isoflurane group, the sevoflurane group mice had shorter anesthetic induction(66.40 ± 2.90S vs. 125.10 ± 6.30S P < 0.0001) and recovery times(28.00 ± 1.07S vs. 56.88 ± 4.14S, P < 0.0001), lower incidence of respiratory depression (0 % vs. 50.00 %, P = 0.0325), and more successful models (93.33 % vs. 60.00 %, P = 0.0801). There were no significant differences in cardiac function, infarction area(49.41 ± 4.18 % vs. 48.66 ± 3.79 %, P = 0.5266), or inflammatory factors in the myocardial infarction area between the two groups. Sevoflurane may therefore be a better choice for the establishment of AMI models in mice.
Insights
Sevoflurane offers faster induction and recovery for creating acute myocardial infarction (AMI) models in mice compared to isoflurane. This anesthetic choice resulted in fewer complications and a higher success rate for AMI model establishment.
Area of Science:
- Anesthesiology
- Cardiovascular Research
- Preclinical Models
Background:
- Anesthetic selection is critical for establishing reliable preclinical models of acute myocardial infarction (AMI).
- Direct comparative studies on sevoflurane and isoflurane for murine AMI models are lacking.
- Understanding anesthetic effects is crucial for optimizing experimental outcomes.
Purpose of the Study:
- To compare the efficacy and safety of sevoflurane versus isoflurane in establishing a murine model of acute myocardial infarction (AMI).
- To evaluate the impact of these anesthetics on anesthetic induction and recovery times, respiratory function, and model success rates.
- To assess the effects on cardiac function and myocardial infarction characteristics.
Main Methods:
- Establishment of a murine AMI model.
- Administration of either sevoflurane or isoflurane for anesthesia.
- Assessment of anesthetic induction and recovery times.
- Evaluation of respiratory depression incidence.
- Confirmation of AMI model success using echocardiography, TTC staining, and PCR analysis.
- Comparison of cardiac function and myocardial infarction area between anesthetic groups.
Main Results:
- Sevoflurane group exhibited significantly shorter anesthetic induction (66.40 ± 2.90s vs. 125.10 ± 6.30s) and recovery times (28.00 ± 1.07s vs. 56.88 ± 4.14s) compared to isoflurane.
- The sevoflurane group demonstrated a significantly lower incidence of respiratory depression (0% vs. 50.00%).
- Model success rates were higher in the sevoflurane group (93.33% vs. 60.00%), although not statistically significant (P=0.0801).
- No significant differences were observed in cardiac function, infarct size (49.41 ± 4.18% vs. 48.66 ± 3.79%), or inflammatory factors between groups.
Conclusions:
- Sevoflurane is a potentially superior anesthetic agent for the establishment of AMI models in mice.
- Its use is associated with improved anesthetic handling characteristics and reduced respiratory complications.
- Further research may validate sevoflurane as the preferred anesthetic for this preclinical model.
