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Microscope-Free Analyte Detection Based on Fiber-Optic Gliding Motility Assays.

Henry Carey-Morgan1, Nabarun Polley2, Till Korten3

  • 1B CUBE - Center for Molecular Bioengineering, TUD University of Technology Dresden, 01307, Dresden, Germany.

Small (Weinheim an Der Bergstrasse, Germany)
|April 16, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel lab-on-fiber device for rapid, cost-effective point-of-care diagnostics. It utilizes motor-protein-driven assays for sensitive detection of biomarkers like Creatine Kinase-MB.

Keywords:
antibodieskinesinlabel‐free detectionmicrotubuleoptical fiber

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Prolonged hospital waiting times increase patient mortality and institutional costs.
  • Current point-of-care diagnostic tools lack cost-effectiveness and broad analyte detection capabilities.
  • Lab-on-fiber technology offers real-time feedback for in situ diagnosis, treatment, and monitoring.

Purpose of the Study:

  • To develop a cost-effective, lab-on-fiber device for rapid, in situ, multiplex analyte detection.
  • To demonstrate proof of concept using motor-protein-driven microtubule assays for molecular detection.
  • To enable microscope-free determination of microtubule gliding speeds for diagnostic purposes.

Main Methods:

  • Harnessing motor-protein-driven microtubule molecular detection assays coupled to optical fibers.
  • Developing a novel method for microscope-free microtubule gliding speed determination.
  • Utilizing antibody-functionalized microtubules for label-free detection of specific analytes.

Main Results:

  • Demonstrated detection of Monomeric Streptavidin and Neutravidin via changes in microtubule gliding speed and bundling.
  • Achieved label-free, microscope-free detection of the heart attack marker Creatine Kinase-MB.
  • Successfully detected secondary antibodies at nanometer concentrations.

Conclusions:

  • The developed lab-on-fiber detector shows potential for in situ, rapid, and low-cost multiplex analyte screening.
  • This technology can significantly improve point-of-care diagnostics, reducing hospital waiting times and costs.
  • The microscope-free detection method offers a practical approach for real-time medical monitoring.