Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

296
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
296
Ion Exchange01:17

Ion Exchange

401
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
401
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

269
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
269
Electrophoresis: Overview01:20

Electrophoresis: Overview

570
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
570
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

431
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
431
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

391
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
391

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery of a novel mitophagy inducer attenuating postoperative cognitive dysfunction through structure-based virtual screening and biological validation.

Free radical biology & medicine·2025
Same author

The Effectiveness of Virtual Walking Intervention in Enhancing Wellness: A Scoping Review.

Studies in health technology and informatics·2025
Same author

Chatbot-Delivered Interventions on Psychological Health Among Young People: A Systematic Review and Meta-Analysis.

Studies in health technology and informatics·2025
Same author

Effectiveness of Ecological Momentary Interventions on Pain, Mental Health, and Quality of Life in Individuals With Rheumatic Diseases: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Journal of nursing management·2025
Same author

Unmasking the rising global burden of depression: A 32-year GBD analysis of gender disparities and regional hotspots in Sub-Saharan Africa.

PloS one·2025
Same author

The Power of Drosophila in Modeling Cancer Cachexia.

Advances in experimental medicine and biology·2025
Same journal

Polymer fatigue: mechanism, mechanics and design.

Materials horizons·2026
Same journal

Bioinspired ultra-fast dissolving core-sheath beaded-structured nanofibrous membranes <i>via</i> one-step emulsion electrospinning for skin moisturization.

Materials horizons·2026
Same journal

A synergistic bio-electro-topological strategy based on an MXene/silk fibroin hydrogel and electrical stimulation for diabetic wound healing.

Materials horizons·2026
Same journal

Relativistic spin-momentum locking in ferromagnets.

Materials horizons·2026
Same journal

Monolithic additive manufacturing of a fluid-structure coupled architected cellular mechanical system for rate-adaptive enhanced energy dissipation.

Materials horizons·2026
Same journal

Decoupling parameters of adhesion from viscoelasticity in the human perception of stickiness <i>via</i> shear-stiffening elastomers.

Materials horizons·2026
See all related articles

Related Experiment Video

Updated: May 13, 2025

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

33.5K

Polyelectrolyte complex-based materials for separations: progress, challenges and opportunities.

Jiaying Li1, Lijie Li1,2, Hestie A Brink2

  • 1Department of Molecules and Materials, Faculty of Science and Technology, MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. s.lindhoud@utwente.nl.

Materials Horizons
|April 16, 2025
PubMed
Summary
This summary is machine-generated.

Polyelectrolyte complex materials offer sustainable separation solutions. Understanding complexation parameters is key to reproducible production and advanced material design for diverse applications.

More Related Videos

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

10.6K
Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

8.4K

Related Experiment Videos

Last Updated: May 13, 2025

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

33.5K
Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

10.6K
Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

8.4K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Separation Technology

Background:

  • Polyelectrolyte complexes (PECs) present a sustainable alternative to conventional materials, particularly in separation technologies.
  • Reproducible production of PEC-based materials is hindered by numerous parameters influencing the complexation process and final material properties.

Purpose of the Study:

  • To provide an overview of how various parameters influence polyelectrolyte complexation.
  • To discuss promising PEC-based materials for separation applications.
  • To identify challenges and opportunities for advancing PEC material development.

Main Methods:

  • Literature review and synthesis of existing research on polyelectrolyte complexation.
  • Analysis of parameters affecting PEC formation and material properties.
  • Discussion of applications including porous membranes, coatings, adhesives, saloplastics, and extraction media.

Main Results:

  • Identified key parameters influencing PEC formation and performance.
  • Highlighted diverse applications of PECs in separation technologies.
  • Outlined current challenges and future opportunities in the field.

Conclusions:

  • Further research is needed to overcome challenges in reproducible PEC production.
  • Collaboration between experimentalists and theoreticians is crucial for advancing PEC material design.
  • Integration of multiscale modeling, machine learning, and AI can accelerate the design and prediction of PEC material properties.