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Efficient Extraction of White Blood Cells Using Modular Inertial Microfluidics
Mingqi Yang1, Chen Ni1, Dan Wu2
1School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China.
Analytical Chemistry
|April 16, 2025
Summary
Modular inertial microfluidics efficiently extract white blood cells (WBCs) from lysed blood. This innovative system offers flexible washing and concentration modules, achieving high recovery rates and outperforming traditional centrifuges.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Cell Separation Technology
Background:
- Massive red blood cell (RBC) presence complicates efficient white blood cell (WBC) extraction.
- Traditional methods like centrifugation face challenges in purity and efficiency for WBC isolation.
Purpose of the Study:
- To develop and validate a modular inertial microfluidic system for efficient WBC extraction from lysed blood.
- To offer a flexible platform adaptable to various sample volumes and operational requirements.
Main Methods:
- Modular inertial microfluidics incorporating washing and concentration modules.
- Optimization of flow rates for different channel sizes within the modules.
- Development of three distinct operational modes by combining modules for diverse applications.
Main Results:
- All three proposed modes demonstrated replacement efficiency and recovery rates exceeding 93%.
- Mode 3 achieved 97.1% replacement efficiency and 95.9% recovery rate.
- The microfluidic system showed superior washing performance with fewer residual RBC fragments compared to centrifuges.
Conclusions:
- Modular inertial microfluidics provide a versatile and efficient solution for WBC extraction from lysed blood.
- The system's adaptability through modular combinations meets diverse processing needs.
- This technology offers improved purity and performance over conventional centrifugation methods.
