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Related Experiment Video

Updated: May 13, 2025

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
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Oscillating microbubble array-based metamaterials (OMAMs) for rapid isolation of high-purity exosomes.

Xinjia Li1,2, Zhiting Deng1, Wenjun Zhang1,3

  • 1Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen 518055, China.

Science Advances
|April 16, 2025
PubMed
Summary

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Researchers developed oscillating microbubble array-based metamaterials (OMAMs) for rapid, label-free isolation of high-purity exosomes from blood. This breakthrough enables efficient exosome subpopulation separation for disease diagnosis and treatment.

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Nanotechnology and Materials Science
  • Cell Biology and Extracellular Vesicles

Background:

  • Exosomes are crucial biomarkers and therapeutic agents, but their isolation from biofluids like blood is difficult.
  • Current methods often require labeling or preprocessing, hindering rapid, high-purity isolation of exosomes and their subpopulations.

Purpose of the Study:

  • To present oscillating microbubble array-based metamaterials (OMAMs) for rapid, label-free isolation of high-purity exosomes and subpopulations.
  • To demonstrate the efficiency of OMAMs in processing undiluted whole blood for exosome purification.

Main Methods:

  • Utilized acoustically excited microbubble oscillation within OMAMs to create acoustofluidic traps.
  • Employed micro/nanoparticle filtering to remove larger bioparticles from biofluids.

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  • Tuned microbubble oscillation amplitude to isolate exosome subpopulations based on size.

    • Main Results:

    • Achieved high-purity (93%) exosomes from undiluted whole blood in approximately 3 minutes.
    • Demonstrated isolation of distinct exosome subpopulations by controlling microbubble oscillation amplitude.
    • OMAMs generate complex acoustic energy patterns using oscillating microbubbles as subwavelength acoustic amplifiers.

    Conclusions:

    • OMAMs offer a rapid, efficient, and label-free method for isolating high-purity exosomes and subpopulations from biofluids.
    • This technology has significant potential for advancing exosome-based disease diagnosis and therapeutic applications.
    • The tunable acoustic properties of OMAMs open avenues for precise manipulation of micro/nanoparticles.