Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Precipitation and Co-precipitation

Precipitation and Co-precipitation

Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...

Sample Preparation for Analysis: Advanced Techniques

Sample Preparation for Analysis: Advanced Techniques

Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...

Atomic Absorption Spectroscopy: Lab

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the...

Sample Preparation for Analysis: Overview

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...

Sublimation

Sublimation

Sublimation is the direct transformation of a solid to a gaseous state. For instance, at standard pressure and room temperature, solid carbon dioxide sublimes to gaseous carbon dioxide. The phase diagram depicts the conditions required for sublimation. This process occurs at the solid-gas phase boundary and is not observed above the triple point of the substance. The reverse of sublimation is called deposition, where a gaseous substance condenses directly into a solid. Sublimation and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of a Hydraulic-Drive Automatic Column Chromatography System for High Precision Isotope Analysis.

Analytical chemistry·2026

Same author

Click-Chemistry-Based Antibacterial Hybrid Hydrogel with Sustained Puerarin Release for Diabetic Wound Healing.

ACS applied bio materials·2026

Same author

Intranasal DNA nanocarrier vaccines with surface-patterned antigens enhance efficacy against respiratory syncytial virus.

Nature materials·2026

Same author

Extreme mass-independent fractionation of mercury isotopes driven by atmospheric plasma-induced chemical process.

Nature communications·2026

Same author

Study on the Effects of Steel Slag on the Mechanical Properties and Microstructure of Cement-Stabilised Base Course Mixtures.

Materials (Basel, Switzerland)·2026

Same author

Reaction mechanisms and microstructural development of MSWI fly ash in geopolymers enhanced by mechanochemical activation.

Waste management (New York, N.Y.)·2026

Search research articles

Related Experiment Video

Updated: May 13, 2025

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Published on: September 1, 2017

Hydride Generation-Based Purification Method for High-Precision Antimony Isotopic Analysis in Low-Concentration and

Shuyang Li^1,2, Junhang Dong^1,2, Linjie Chen¹

¹State Key Laboratory of Geomicrobiology and Environmental Changes, China University of Geosciences, Wuhan 430074, China.

Analytical Chemistry

|April 16, 2025

Summary

A new two-step purification method using hydride generation (HG) enables precise antimony (Sb) isotope analysis in challenging environmental samples. This rapid technique accurately traces Sb sources and geochemical behavior in complex matrices.

More Related Videos

Separation of Uranium and Thorium for 230Th-U Dating of Submarine Hydrothermal Sulfides

Separation of Uranium and Thorium for 230Th-U Dating of Submarine Hydrothermal Sulfides

Published on: May 20, 2019

Sulfate Separation by Selective Crystallization with a Bis-iminoguanidinium Ligand

Sulfate Separation by Selective Crystallization with a Bis-iminoguanidinium Ligand

Published on: September 8, 2016

Related Experiment Videos

Last Updated: May 13, 2025

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation - Atomic Absorption Spectrometry.

Published on: September 1, 2017

Separation of Uranium and Thorium for 230Th-U Dating of Submarine Hydrothermal Sulfides

Separation of Uranium and Thorium for 230Th-U Dating of Submarine Hydrothermal Sulfides

Published on: May 20, 2019

Sulfate Separation by Selective Crystallization with a Bis-iminoguanidinium Ligand

Sulfate Separation by Selective Crystallization with a Bis-iminoguanidinium Ligand

Published on: September 8, 2016

Area of Science:

Geochemistry
Environmental Science
Analytical Chemistry

Background:

Antimony (Sb) isotopes are crucial for tracing Sb sources and understanding its geochemical behavior.
Accurate determination of Sb isotopic compositions (δ¹²³Sb) is challenging in low-concentration, complex natural samples.

Purpose of the Study:

To introduce a novel two-step purification method for high-precision Sb isotope analysis.
To enable accurate and precise Sb isotopic analysis in low-Sb environmental samples.

Main Methods:

A two-step purification process involving hydride generation (HG) and a thiol silica column.
Optimized HG purification with distinct flow and no-flow modes for water and solid samples, respectively.
Validation using Sb standard solutions and geochemical reference samples.

Main Results:

Complete Sb purification recovery with effective matrix element removal and low procedure blanks (<0.1 ng).
Rapid purification times: 1 hour for water samples and 1.5 hours for geological samples.
δ¹²³Sb values in excellent agreement with reference data, indicating no isotopic fractionation.

Conclusions:

The novel HG-based method provides a rapid, efficient, and accurate strategy for high-precision Sb isotopic analysis.
Successfully applied to diverse low-Sb environmental samples (river, seawater, sediments).
Enhances the investigation of Sb isotopic reservoirs within biogeochemical cycles.