Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Induced-fit Model

Induced-fit Model

Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Metabolic Engineering of Microbial Cell Factories for Sustainable Production of Glycolic Acid: Pathways, Chassis, and Strategies toward Poly(glycolic acid) Bioplastics.

ACS synthetic biology·2026

Same author

Biofilm-forming traits enrich the plasmid diversity and functional potential in particle-attached bacteria in coastal ecosystems.

Microbiology spectrum·2026

Same author

Surface-based brain age index reflects hematological impacts on cognitive development in children with beta-thalassemia major.

iScience·2026

Same author

Sustainable Production of Ferulic Acid and Prebiotic Sugars via Spore-Displayed Bifunctional Enzymes Purified through Integrated ELP-Intein Strategies.

Journal of agricultural and food chemistry·2026

Same author

Functional interaction between Bacillus velezensis D103 and maize reveals a microbial mechanism for drought stress mitigation via photosynthesis and sugar metabolism.

Plant science : an international journal of experimental plant biology·2026

Same author

L-xylulose, a new versatile rare sugar: recent biosynthetic advances and challenges.

Food research international (Ottawa, Ont.)·2026

Search research articles

Related Experiment Video

Updated: May 13, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Engineered interaction elements enable enhanced multi-enzyme assembly and cascade biocatalysis for indigo synthesis.

Shumin Xu¹, Gao Song¹, Xianghui Qi²

¹College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, China; Shenyang Key Laboratory of Microbial Resource Mining and Molecular Breeding, China.

Bioresource Technology

|April 16, 2025

Summary

New peptide and protein interaction systems (PB1C/PB2N and importin/PB2C) enable efficient multi-enzyme assembly for enhanced metabolic flux control and improved biosynthetic pathway yields, such as doubling indigo production.

Keywords:

Biocatalysis Cascade reaction Metabolic engineering Scaffold multienzyme system Synthetic biology

More Related Videos

Bridging the Bio-Electronic Interface with Biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

Related Experiment Videos

Last Updated: May 13, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Bridging the Bio-Electronic Interface with Biofabrication

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

Area of Science:

Biocatalysis and Metabolic Engineering
Synthetic Biology
Protein Engineering

Background:

Enzyme scaffolds facilitate multi-enzymatic cascade reactions by enabling substrate channeling and controlling metabolic flux.
Limited availability of protein-protein interaction elements restricts the widespread application of enzyme assembly strategies.

Purpose of the Study:

To develop novel peptide-peptide (PB1C/PB2N) and protein-peptide (importin/PB2C) interaction systems for protein assembly.
To evaluate the efficacy of these new systems, alongside the existing RIDD/RIAD pair, in enhancing multi-enzymatic cascade reactions.
To optimize the indigo synthesis pathway using these developed scaffold systems.

Main Methods:

Fusion of PB1C/PB2N and importin/PB2C pairs to target proteins to induce self-assembly.
Integration of these novel interaction elements with the RIDD/RIAD system for enhanced cascade biocatalysis.
Optimization of the indigo synthesis pathway via engineered enzyme assembly.

Main Results:

Demonstrated successful protein assembly using the novel PB1C/PB2N and importin/PB2C interaction systems.
Achieved a twofold increase in indigo yield through interaction element-based cascade biocatalysis compared to traditional co-expression.
Showcased improved control over metabolic flux and pathway efficiency.

Conclusions:

PB1C/PB2N and importin/PB2C systems represent valuable tools for constructing enzyme scaffolds.
These systems significantly enhance the efficiency of multi-enzymatic cascade reactions and biosynthetic pathways.
The developed scaffold systems offer a promising approach for metabolic engineering and synthetic biology applications.