Related Experiment Video
Updated: May 11, 2025
Author Spotlight: Exploring Bradysia coprophila's Unique Biology – A Guide to Laboratory Maintenance
Published on: April 19, 2024
Deep Insight into the genome of a major rice pest, Scripophaga incertulas
Bingbing He1, Zhangqi Zuo1, Hongxing Xu2
1State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects & Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Abstract:
The yellow stem borer, Scripophaga incertulas (Crambidae, Lepidoptera), is a highly destructive pest damaging rice crops. Here, we combined Illumina, PacBio sequencing and Hi-C scaffolding to generate a high-quality chromosome-level genome assembly of S. incertulas. We initially obtained an 839.78 Mb assembly with scoffold N50 of 1.13 Mb. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis demonstrated that this genome assembly has a high-level completeness of 97.5% gene coverage. The assembly contained 45.67% repetitive sequences (383.50 Mb), and has been annotated with 19,874 protein-coding genes. Next, 3,369 scaffolds were anchored to 26 chromosomes, resulting in an increase of the N50 to 32.16 Mb. Our research provides insights into the evolution and ecology of S. incertulas, offering valuable resources for future pest management.
