Evaluation of sequential glucose metabolism in cerebral ischemia using a chrono-autoradiographic method

A chrono-autoradiographic technique (sequential double label 2-deoxyglucose method) was performed to evaluate postischemic regional glucose metabolism. Using this method, two sequential autoradiograms, one immediately after the stroke and one 7-8 hr after the stroke, were obtained from the same brain slice. Comparison of the two images strongly suggested that the ischemic core and the regions adjacent to the core died, while the periphery recovered from the metabolic injury, and that postischemic metabolic abnormality itself didn't necessarily determine the fate of the ischemic tissue.