Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

72.8K
Overview
72.8K
Multiple Allele Traits01:49

Multiple Allele Traits

33.8K
The Concept of Multiple Allelism
33.8K
Epistasis Analysis01:09

Epistasis Analysis

4.8K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
4.8K
Allosteric Regulation01:08

Allosteric Regulation

57.2K
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
57.2K
Genetic Lingo01:11

Genetic Lingo

98.4K
Overview
98.4K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

32.8K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
32.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biometeorological regulation of male and female fertility traits in banana (Musa spp.) across contrasting flowering environments.

Scientific reports·2026
Same author

Quantitative Gene Expression Analysis Across Contrasting Linseed Genotypes Identifies New Candidates for Aluminum Toxicity and Low-Phosphorus Tolerance.

Plant, cell & environment·2026
Same author

Ascertaining the morpho-molecular diversity in buckwheat germplasm and identification of high yielding, stable genotypes with superior biochemical quality.

Scientific reports·2025
Same author

A simple seed-piercing transformation protocol for pearl millet and finger millet.

AoB PLANTS·2025
Same author

Metabolite profiling reveals differential accumulation of secondary metabolites related to flavour and colour across four heirloom chilli landraces.

Physiology and molecular biology of plants : an international journal of functional plant biology·2025
Same author

Keloid: a rare clinical image.

The Pan African medical journal·2025

Related Experiment Video

Updated: May 11, 2025

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

22.8K

Allelic value in gene regulation-implications for gene editing.

Mazahar Moin1, Mayank Rai2, Wricha Tyagi1

  • 1International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, Telangana, India.

Annals of Botany
|April 17, 2025
PubMed
Summary

Gene editing precisely modifies plant traits, but success depends on targeting the right gene alleles within specific genetic backgrounds. Functional validation across diverse backgrounds is crucial for predictable outcomes in crop improvement.

Keywords:
Allelesgenetic backgroundphenotypericetomatotrait

More Related Videos

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
11:31

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

13.9K
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

413

Related Experiment Videos

Last Updated: May 11, 2025

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

22.8K
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
11:31

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

13.9K
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

413

Area of Science:

  • Plant genetics and breeding
  • Molecular biology
  • Agricultural science

Background:

  • Gene editing is a precise tool for plant trait modification, yet understanding optimal targets and genetic backgrounds for desired phenotypes remains a challenge.
  • Extensive gene editing data in tomato and rice provide a foundation for analyzing trait modification strategies.

Purpose of the Study:

  • To analytically overview targeted traits in gene-edited crops, particularly tomato and rice, to understand advancements and bottlenecks.
  • To assess the potential of gene editing for de novo domestication, grain quality, fruit color, yield, and stress tolerance by analyzing existing data.

Main Methods:

  • Revisiting available gene editing data on allelic values within molecular pathways in tomato and rice.
  • Assessing and comparing phenotypes of edits across different genetic backgrounds with resequencing and phenotypic data.
  • Evaluating traits including de novo domestication, grain quality, fruit color, yield, and stress tolerance.

Main Results:

  • Gene editing effectiveness is significantly influenced by the roles of generated alleles in regulating genetic pathways.
  • Phenotypic outcomes of gene editing vary considerably across different genetic backgrounds, with some lines showing desired traits and others exhibiting pleiotropic effects or no expected phenotype.
  • The influence of an allele on a trait depends on the trait's nature, gene position in a pathway, and the genetic background.

Conclusions:

  • Functional validation of targeted alleles across multiple distinct genetic backgrounds is essential to confirm their utility.
  • Prioritizing identification of suitable candidate genes and regulatory motifs is recommended over direct intervention in coding sequences to address challenges in achieving desired phenotypes.
  • Gene editing can lead to diverse phenotypic variations, highlighting the critical role of genetic background in trait modification.