Procedure for the isolation of mouse anti H-2 antibodies from complex alloantisera

B6AF1-anti-B10.D2 ascites fluid was incubated with donor strain red blood cells to absorb the anti-H-2 antibodies. The antibody-coated RBC were then lysed, and from the ghosts the antibodies were eluted. This anti-H-2 preparation was further purified by affinity chromatography, using an immobilized anti IgG2 antiserum. Thus a pure anti-H-2 IgG2 was obtained for in vitro and in vivo testing.