[Chemiluminescent determination of iron, copper and cobalt in the serum]

Iron in the presence of diethylenetriamine, an activator, is a catalyst of the chemiluminescent reaction between 4-diethylaminophthalhydraside and hydrogen peroxide. The following conditions are optimal for iron content determination: concentration of 4-diethylaminophthalhydraside - (4-5) X 10(-5) M, H2O2 - 1 X 10(-3) M, diethylenetriamine - 5 X 10(-4) M; pH 10-11. Chemoluminescent reaction between 4-diethylaminophthalhydrazide and hydrogen peroxide is catalyzed also by copper and cobalt ions. This reaction is studied for the possibility to be used to determine iron, copper and cobalt amount in solution without their preliminary separation. A highly sensitive procedure is developed for chemiluminescent determination of these metals concentration in the blood serum with detection limit for iron 1.0, for copper - 0.1 and for cobalt - 0.05 ng/ml.