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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
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Storage Temperature Affects Platelet Activation and Degranulation in Response to Stimuli
Ben Winskel-Wood1, Denese C Marks1,2, Lacey Johnson1,3
1Research and Development, Australian Red Cross Lifeblood, Sydney, NSW 2015, Australia.
International Journal of Molecular Sciences
|April 17, 2025
Summary
Cold storage of platelets up to 21 days enhances their ability to activate and release factors when stimulated, compared to room-temperature storage. This suggests improved functionality for transfusion.
Area of Science:
- Hematology
- Transfusion Medicine
- Biotechnology
Background:
- Refrigeration (cold storage) extends platelet shelf-life to 21 days, offering benefits over room-temperature (RT) storage.
- The impact of storage conditions on platelet activation responsiveness remains incompletely understood.
Purpose of the Study:
- To investigate the effect of cold storage versus RT storage on platelet activation and function.
- To compare the response of cold-stored and RT-stored platelets to various stimuli.
Main Methods:
- Paired study comparing buffy-coat platelet concentrates stored at RT or cold temperatures.
- Platelet analysis at 1, 7, 14, and 21 days using imaging flow cytometry and ELISA.
- Stimulation assays with TRAP-6, A23187, lipopolysaccharides, and Histone-H4.
Main Results:
- Cold-stored platelets showed increased procoagulant activity and extracellular vesicle generation upon stimulation.
- Basal levels of soluble factors were lower in cold-stored platelets, indicating better granule retention.
- Cold-stored platelets released more soluble factors after stimulation and uniquely responded to lipopolysaccharides.
Conclusions:
- Cold-stored platelets maintain their capacity to respond to diverse stimuli even after 21 days.
- This preserved responsiveness suggests potential for enhanced functional efficacy post-transfusion.
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